The long-term fate of deposited nitrogen in temperate forest soils

Veerman Liz 1, Kalbitz Karsten 2, Gundersen Per 3, Kjønaas Janne 4, Moldan Filip 5, Schleppi Patrick 6, Van Loon Emiel E. 1, Schoorl Jorien 1, Wessel Wim 1, Tietema Albert 1

1 Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands
2 Institute of Soil Science and Site Ecology, Technische Universität Dresden, Tharandt, Germany
3 Department of Geosciences and Natural Resource Management, University of Copenhagen, Frederiksberg, Denmark
4 Norwegian Institute of Bioeconomy Research (NIBIO), Ås, Oslo, Norway
5 Swedish Environmental Research Institute (IVL), Göteborg, Sweden
6 Swiss Federal Institute for Forest, Snow and Landscape Research (WSL), Birmensdorf, Switzerland
7 Swiss Federal Institute for Forest, Snow and Landscape Research (WSL), Birmensdorf, Switzerland

Biogeochem. 150 (2020): 1-15

DOI: 10.1007/s10533-020-00683-6


Increased anthropogenic nitrogen (N) inputs can alter the N cycle and affect forest ecosystem functions. The impact of increased N deposition depends among others on the ultimate fate of N in plant and soil N pools. Short-term studies (3–18 months) have shown that the organic soil layer was the dominant sink for N. However, longer time scales are needed to investigate the long-term fate of N. Therefore, the soils of four experimental forest sites across Europe were re-sampled ~ 2 decades after labelling with 15N. The sites covered a wide range of ambient N deposition varying from 13 to 58 kg N ha-1N year-1 N. To investigate the effects of different N loads on 15N recovery, ambient N levels were experimentally increased or decreased. We hypothesized that: (1) the mineral soil would become the dominant 15N sink after 2 decades, (2) long-term increased N deposition would lead to lower 15N recovery levels in the soil and (3) variables related to C dynamics would have the largest impact on 15N recovery in the soil. The results show that large amounts of the added 15N remain in the soil after 2 decades and at 2 out of 4 sites the 15N recovery levels are higher in the mineral soil than in the organic soil. The results show no clear responses of the isotopic signature to the changes in N deposition. Several environmental drivers are identified as controlling factors for long-term 15N recovery. Most drivers that significantly contribute to 15N recovery are strongly related to the soil organic matter (SOM) content. These findings are consistent with the idea that much of the added 15N is immobilized in the SOM. In the organic soil layer, we identify C stock, thickness of the organic layer, N-status and mean annual temperature of the forest sites as most important controlling factors. In the mineral soil we identify C stock, C content, pH, moisture content, bulk density, temperature, precipitation and forest stand age as most important controlling factors. Overall, our results show that these temperate forests are capable of retaining long-term increased N inputs preferably when SOM availability is high and SOM turnover and N availability are low.

Keywords: 15N tracer, N deposition, N-status,Long-term, SOM accumulation